p5e ubi (Addgene inc)
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Addgene inc
p5e ubi
P5e Ubi, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p5e+ubi/pENTR5'_ubi+(Plasmid+%2327320)/bio_rxiv__2025__08__20__670374-198-8-9
Average 93 stars, based on 34 article reviews
P5e Ubi, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p5e+ubi/pENTR5'_ubi+(Plasmid+%2327320)/bio_rxiv__2025__08__20__670374-198-8-9
Average 93 stars, based on 34 article reviews
p5e ubi - by Bioz Stars,
2026-10
93/100 stars
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Recombinant:Article Title: PAX3-FOXO1 transgenic zebrafish models identify HES3 as a mediator of rhabdomyosarcoma tumorigenesis Article Snippet: Recombinant DNA reagent , p5E mcs , , , PMID:17937395 , , , , . .. Recombinant DNA reagent , Construct:Article Title: Live Tracking of Inter-organ Communication by Endogenous Exosomes In Vivo. Article Snippet: .. pUbi-CD63-pHluorin was constructed by first cloning CD63-pHluorin (human CD63; sequence identical to Verweij et al., 2018) using primer pairs 5’-GGGGACAAGTTTGTACAAAAAAGCAGGCTGGatggcggtggaaggagga-3’ (fwd) and 5’-GGGGACCACTTTGTACAA GAAAGCTGGGTCctacatcacctcgtagccacttct-3’ (rev) into pDONR221 (Gateway, Invitrogen). pDONR221-CD63-pHluorin was subsequently recombined with Article Title: An optimized QF-binary expression system for use in zebrafish. Article Snippet: .. Tol2-based QF driver constructs: To generate Tol2-based vectors expressing QF variants under the control of the ubi promoter [22], we recombined the following vectors using Gateway LR Clonase II (ThermoFisher): Article Title: Live tracking of inter-organ communication by endogenous exosomes in vivo Article Snippet: BODIPY C12 558/568 (Life Technologies SAS, D3835) was injected in the yolk at 1.5 ng/nl BODIPY C12, using canola oil as lipid carrier. .. Collagenase I was used at 200mg/mL (ThermoFisher, 17018029). pUbi-CD63-pHluorin was constructed by first cloning CD63-pHluorin (human CD63; sequence identical to ) using primer pairs 5′-GGGGACAAGTTTGTACAAAAAAGCAGGCTGGatggcggtggaaggagga-3′ and 5′-GGGGACCACTTTGTACAAGAAAGCTGGGTCctacatcacctcgtagccacttct-3′ into pDONR221 (Gateway, Invitrogen). pDONR221-CD63-pHluorin was subsequently recombined with Cloning:Article Title: Live Tracking of Inter-organ Communication by Endogenous Exosomes In Vivo. Article Snippet: .. pUbi-CD63-pHluorin was constructed by first cloning CD63-pHluorin (human CD63; sequence identical to Verweij et al., 2018) using primer pairs 5’-GGGGACAAGTTTGTACAAAAAAGCAGGCTGGatggcggtggaaggagga-3’ (fwd) and 5’-GGGGACCACTTTGTACAA GAAAGCTGGGTCctacatcacctcgtagccacttct-3’ (rev) into pDONR221 (Gateway, Invitrogen). pDONR221-CD63-pHluorin was subsequently recombined with Article Title: Live tracking of inter-organ communication by endogenous exosomes in vivo Article Snippet: BODIPY C12 558/568 (Life Technologies SAS, D3835) was injected in the yolk at 1.5 ng/nl BODIPY C12, using canola oil as lipid carrier. .. Collagenase I was used at 200mg/mL (ThermoFisher, 17018029). pUbi-CD63-pHluorin was constructed by first cloning CD63-pHluorin (human CD63; sequence identical to ) using primer pairs 5′-GGGGACAAGTTTGTACAAAAAAGCAGGCTGGatggcggtggaaggagga-3′ and 5′-GGGGACCACTTTGTACAAGAAAGCTGGGTCctacatcacctcgtagccacttct-3′ into pDONR221 (Gateway, Invitrogen). pDONR221-CD63-pHluorin was subsequently recombined with Sequencing:Article Title: Live Tracking of Inter-organ Communication by Endogenous Exosomes In Vivo. Article Snippet: .. pUbi-CD63-pHluorin was constructed by first cloning CD63-pHluorin (human CD63; sequence identical to Verweij et al., 2018) using primer pairs 5’-GGGGACAAGTTTGTACAAAAAAGCAGGCTGGatggcggtggaaggagga-3’ (fwd) and 5’-GGGGACCACTTTGTACAA GAAAGCTGGGTCctacatcacctcgtagccacttct-3’ (rev) into pDONR221 (Gateway, Invitrogen). pDONR221-CD63-pHluorin was subsequently recombined with Article Title: Live tracking of inter-organ communication by endogenous exosomes in vivo Article Snippet: BODIPY C12 558/568 (Life Technologies SAS, D3835) was injected in the yolk at 1.5 ng/nl BODIPY C12, using canola oil as lipid carrier. .. Collagenase I was used at 200mg/mL (ThermoFisher, 17018029). pUbi-CD63-pHluorin was constructed by first cloning CD63-pHluorin (human CD63; sequence identical to ) using primer pairs 5′-GGGGACAAGTTTGTACAAAAAAGCAGGCTGGatggcggtggaaggagga-3′ and 5′-GGGGACCACTTTGTACAAGAAAGCTGGGTCctacatcacctcgtagccacttct-3′ into pDONR221 (Gateway, Invitrogen). pDONR221-CD63-pHluorin was subsequently recombined with Plasmid Preparation:Article Title: Live Tracking of Inter-organ Communication by Endogenous Exosomes In Vivo. Article Snippet: .. pUbi-CD63-pHluorin was constructed by first cloning CD63-pHluorin (human CD63; sequence identical to Verweij et al., 2018) using primer pairs 5’-GGGGACAAGTTTGTACAAAAAAGCAGGCTGGatggcggtggaaggagga-3’ (fwd) and 5’-GGGGACCACTTTGTACAA GAAAGCTGGGTCctacatcacctcgtagccacttct-3’ (rev) into pDONR221 (Gateway, Invitrogen). pDONR221-CD63-pHluorin was subsequently recombined with Article Title: Imaging cellular activity simultaneously across all organs of a vertebrate reveals body-wide circuits Article Snippet: .. These included a ubiquitous promoter containing vector, or Article Title: Live tracking of inter-organ communication by endogenous exosomes in vivo Article Snippet: BODIPY C12 558/568 (Life Technologies SAS, D3835) was injected in the yolk at 1.5 ng/nl BODIPY C12, using canola oil as lipid carrier. .. Collagenase I was used at 200mg/mL (ThermoFisher, 17018029). pUbi-CD63-pHluorin was constructed by first cloning CD63-pHluorin (human CD63; sequence identical to ) using primer pairs 5′-GGGGACAAGTTTGTACAAAAAAGCAGGCTGGatggcggtggaaggagga-3′ and 5′-GGGGACCACTTTGTACAAGAAAGCTGGGTCctacatcacctcgtagccacttct-3′ into pDONR221 (Gateway, Invitrogen). pDONR221-CD63-pHluorin was subsequently recombined with Expressing:Article Title: An optimized QF-binary expression system for use in zebrafish. Article Snippet: .. Tol2-based QF driver constructs: To generate Tol2-based vectors expressing QF variants under the control of the ubi promoter [22], we recombined the following vectors using Gateway LR Clonase II (ThermoFisher): Control:Article Title: An optimized QF-binary expression system for use in zebrafish. Article Snippet: .. Tol2-based QF driver constructs: To generate Tol2-based vectors expressing QF variants under the control of the ubi promoter [22], we recombined the following vectors using Gateway LR Clonase II (ThermoFisher): |
![Schematics of the new RNAi backbone for zebrafish gene silencing. ( A ) <t>Tol2kit</t> compatible p3E presenting an optimized empty synthetic pri-miR (or RNAi cassette) embedded into a β-globin intronic sequence. This presented pri-miR is designed to allow rapid directional insertion of synthetic pre-miR of choice. Synthetic p re-miR(s) of choice are generated by annealing specific top and bottom RNAi oligos designed to release a mature miRNA directed against the 3′UTR of a gene-of-interest [see ‘Methods’ section and previous material ]. The RNAi cassette is flanked by restriction sites allowing subsequent and repetitive chaining [see ‘Methods’ and ) for generating p3E-RNAi plasmid with multiple pri-miR for either increasing the potency of the desired knockdown or targeting multiple genes at the same time. ( B ) Without intron, the pre-miR /RNAi cassette is transcribed along with a co-marker on the same RNA, which is cut by Drosha in the nucleus for releasing the associated pre-miR . This cut leaves the mRNA without polyA tail and leads to its rapid degradation. The associated fluorescence is not a good indicator of the activity and amount of synthetic miRNA produced. ( C ) The presence of the intronic sequence is designed to rescue the co-expression of the marker (see also ).](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_4099/pmc11744099/pmc11744099__gkaf004fig1.jpg)